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目的:观察小檗碱和吴茱萸碱配伍对高脂血症模型大鼠肝脏HMG-CoA还原酶和L-FABP基因表达的影响,探讨黄连吴茱萸配伍发挥降脂作用的机制。方法:将40只健康雄性SD大鼠随机分为正常对照组,模型对照组,辛伐他汀组,小檗碱与吴茱萸碱配伍高、低剂量组,每组8只;使用高脂乳剂建立大鼠高脂血症模型,以18sRNA作为内参基因,RT-PCR技术检测各组大鼠肝组织中HMG-CoA还原酶和L-FABP mRNA相对表达水平。结果:小檗碱和吴茱萸碱配伍能显著降低高脂血症模型大鼠肝脏HMG-CoA还原酶和L-FABP mRNA表达,且高剂量组效果更优。结论:黄连吴茱萸降血脂有效成分小檗碱、吴茱萸碱配伍可降低高脂血症模型大鼠的血脂水平,其作用机制可能与抑制肝脏HMG-CoA还原酶和L-FABP基因表达有关。
Objective: To observe the effects of berberine and evodiamine compatibility on HMG-CoA reductase and L-FABP gene expression in the liver of hyperlipidemic rats, and to explore the mechanism of compatibility of Evodia rutaecarpa and Fructus Evodia to exert lipid-lowering effect. Methods: Forty healthy male Sprague-Dawley rats were randomly divided into normal control group, model control group, simvastatin group, berberine and evodiamine compatibility high and low dose group, each group of eight; the use of high-fat emulsion to establish large Rat hyperlipidemia model, 18sRNA was used as a reference gene, and the relative expression levels of HMG-CoA reductase and L-FABP mRNA were detected by RT-PCR in each group. Results: The combination of berberine and evodiamine significantly reduced the expression of HMG-CoA reductase and L-FABP mRNA in the liver of hyperlipidemic rats, and the high dose group was more effective. CONCLUSION: The effective components of berberine and evodiamine can decrease the lipid level of hyperlipemia model rats. The possible mechanism may be related to inhibiting the expression of HMG-CoA reductase and L-FABP in the liver.