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[目的]本文旨在研究tetraspanin3基因在抗番茄黄化曲叶病毒(TYLCV)病中的作用及对番茄生长发育的影响。[方法]通过MEG 5.0软件构建进化树,分析tetraspanin3基因的进化;采用实时荧光定量PCR(RT-q PCR)检测tetraspanin3在番茄各个器官中的表达量及对TYLCV诱导的响应;通过病毒诱导基因沉默技术(VIGS)将番茄中的tetraspanin3沉默,测定基因沉默后番茄的抗病性并观测沉默植株表型。[结果]构建的进化树显示:番茄的tetraspanin3基因与葡萄同源基因在一个独立的进化分支上。tetraspanin3基因在各器官中均有表达,在茎、老叶、花中表达量较高,在根、果实中表达量较低,在新叶中的表达量处于中间水平。tetraspanin3受TYLCV诱导后上调表达。沉默tetraspanin3后的植株出现了叶片扭曲,并且在接种TYLCV后,TYLCV病毒量降低50%以上。[结论]tetraspanin3与番茄叶片的生长发育有关,并且对TYLCV侵染番茄植株起正调控作用。
[Objective] The aim of this study was to investigate the effect of tetraspanin3 gene on the resistance to TYLCV and its effect on tomato growth and development. [Method] The phylogenetic tree was constructed by MEG 5.0 software to analyze the evolution of tetraspanin3 gene. The expression of tetraspanin3 in various organs of tomato and its response to TYLCV were detected by real-time fluorescence quantitative PCR (RT-qPCR) Technology (VIGS) Silencing tetraspanin3 in Tomatoes to Determine Tomato Disease Resistance After Silencing and To Determine Silent Plant Phenotype. [Result] The constructed phylogenetic tree showed that the tomato tetraspanin3 gene and the grape homologous gene were on an independent evolutionary branch. The tetraspanin3 gene was expressed in all organs, with high expression in stem, old leaves and flowers, lower expression in roots and fruits, and intermediate expression in new leaves. Tetraspanin3 is upregulated after being induced by TYLCV. Plants following silencing of tetraspanin3 exhibited leaf distortions and TYLCV virus levels were reduced by more than 50% after vaccination with TYLCV. [Conclusion] tetraspanin3 is related to the growth and development of tomato leaves and plays a positive regulatory role in tomato plants infected with TYLCV.