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目的:通过观察不同中药复方对甲状腺功能减退症(简称甲减)肾损害大鼠肾脏功能及形态的影响,探求甲减肾损害大鼠的主要证候属性。方法:将Wistar大鼠随机分为正常组和实验组,实验组建立甲减肾损害大鼠模型后,随机分为模型组、补中益气汤组、逍遥散组和金匮肾气丸组,并施以相应处理因素。90天后,测定血清甲状腺激素(TT3、TT4)、血清促甲状腺激素(TSH)、24h尿白蛋白、血清肌酐(Scr)和血清尿素氮(BUN),观察肾脏结构。结果:①24h尿白蛋白、Scr和BUN:补中益气汤组高于正常组(P<0.05),低于模型组(P<0.01),低于逍遥散组和金匮肾气丸组(P<0.05);逍遥散组和金匮肾气丸组低于模型组(P<0.05)。②模型组肾小球体积增大,系膜区增宽、系膜细胞及系膜基质增生;3个治疗组病理改变均有不同程度减轻,且补中益气汤组改善显著。结论:在甲减肾损害机体内,补中益气汤可较好地改善肾脏功能及形态,从而推测脾气虚弱在甲减肾损害发病中起重要作用。
OBJECTIVE: To observe the effects of different Chinese herbal formulas on renal function and morphology in rats with hypothyroidism (hypothyroidism) and to explore the main syndrome properties of rats with hypothyroidism. Methods: Wistar rats were randomly divided into normal group and experimental group. After the experimental group was established rat model of hypothyroidism, they were randomly divided into model group, Buzhong Yiqi Decoction group, Xiaoyao San group and Jinkui Shenqi Pill group. , and impose corresponding treatment factors. After 90 days, serum thyroid hormone (TT3, TT4), serum thyroid stimulating hormone (TSH), 24h urinary albumin, serum creatinine (Scr), and serum urea nitrogen (BUN) were measured and the kidney structure was observed. Results: The urinary albumin, Scr and BUN: Buzhong Yiqi Decoction group was higher than normal group (P<0.05), lower than the model group (P<0.01), and lower than Xiaoyao San group and Jinkui Shenqi Pill group (P<0.01). P<0.05); Xiaoyaosan group and Jinkuishenqiwan group were lower than model group (P<0.05). 2In the model group, the glomerular volume increased, mesangial area broadened, mesangial cells and mesangial matrix hyperplasia; pathological changes in the three treatment groups all decreased to varying degrees, and the Buzhong Yiqi Decoction group improved significantly. Conclusion: Buzhong Yiqi Decoction can improve renal function and morphology in the body of hypothyroidism kidney damage. It is speculated that spleen deficiency plays an important role in the pathogenesis of hypothyroidism.