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恶性黑色素瘤约占恶性肿瘤的1%,仅次于肺癌,外检诊断常遇困难,与癌、淋巴瘤和肉瘤难以区别。过去常用嗜银染色或多巴氧化酶组化技术,但不理想。近年来有人用抗S-100蛋白单克隆抗体免疫细胞化学诊断黑色素瘤。这种方法敏感,但无特异性。本文介绍一种单克隆抗体HMB-45,在固定包埋组织中对黑色素瘤和交界痣有高度敏感性及绝对特异性。取含有黑色素瘤转移的腋窝淋巴结作抗原免疫BALB/C小鼠,制作单克隆抗体HMB-45。选取18例原发性皮肤黑色素
Malignant melanoma accounts for about 1% of malignant tumors, second only to lung cancer. The diagnosis of external melanoma often encounters difficulties and is indistinguishable from cancer, lymphoma and sarcoma. In the past, argyrophil staining or dopa oxidase histochemistry was used, but it was not ideal. In recent years, the use of anti-S-100 protein monoclonal antibody immunocytochemistry for the diagnosis of melanoma. This method is sensitive but not specific. This article describes a monoclonal antibody, HMB-45, that has high sensitivity and absolute specificity for melanoma and borderline fistulas in fixed-embedded tissues. BALB/C mice were immunized with axillary lymph nodes containing melanoma metastasis as antigens to produce monoclonal antibody HMB-45. Select 18 cases of primary skin melanin