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为探索小麦水通道蛋白在氮素处理过程中的生物学功能,以0.1%尿素处理6.0 h的周麦19幼根为材料,利用RT-PCR方法克隆了小麦PIPs类型的水通道蛋白基因TaPIP1,并分析了该基因的组织表达特性及其在尿素处理过程中的表达特征。TaPIP1全长1 062 bp,包括61 bp的5′非翻译区,128 bp的3′非翻译区和一个长度为873 bp、编码290个氨基酸的开放阅读框。序列分析表明,TaPIP1基因与已知小麦(GQ452384和AAM00368)、大麦(BAA23746,CAA54233和BAA23745)、水稻(BAA24016)和玉米(AAK26754,ACG39699,ACG37183,CAA57955和AAK26756)等单子叶植物来源的同类基因同源性较高,相似性为85.9%~99.3%。半定量RT-PCR表达谱分析显示,TaPIP1在抽穗期小麦的根、茎和旗叶中均能表达。氮素处理下该基因表达分析结果显示,TaPIP1在萌发期小麦根系中的表达受尿素的诱导。
In order to explore the biological function of wheat aquaporin during nitrogen treatment, the PQs-type aquaporin gene TaPIP1 was cloned by RT-PCR using 0.1% urea-treated 6.0% The tissue expression characteristics of the gene and its expression characteristics during urea treatment were analyzed. TaPIP1 is 1 062 bp in length and consists of 61 bp of 5 ’untranslated region, 128 bp of 3’ untranslated region and an open reading frame of 873 bp encoding 290 amino acids. Sequence analysis showed that the TaPIP1 gene is homologous to monocotyledonous genes such as wheat (GQ452384 and AAM00368), barley (BAA23746, CAA54233 and BAA23745), rice (BAA24016) and maize (AAK26754, ACG39699, ACG37183, CAA57955 and AAK26756) High homology, similarities of 85.9% to 99.3%. Semi-quantitative RT-PCR analysis showed that TaPIP1 was expressed in the roots, stems and flag leaves of wheat at heading. The results of gene expression analysis under nitrogen treatment showed that the expression of TaPIP1 in the root system of wheat during germination was induced by urea.