论文部分内容阅读
目的:建立大豆中5种除草剂多残留同时检测的高效液相色谱分析方法。方法:样品经甲醇提取,C18固相萃取小柱净化后,采用高效液相色谱方法分离,检测波长为230 nm。结果:5种除草剂(普杀特、苯达松、氟磺胺草醚、喹禾灵、2,4-滴丁酯)在0.5~10.0 mg/L范围内线性良好,回收率为77.9%~101.8%,相对标准偏差为1.24%~6.50%,方法的定量限为0.05~0.08 mg/kg,能达到国家有关上述除草剂残留限量的要求。结论:本法操作简便,准确可靠,为质量监控提供了参考。
Objective: To establish a high performance liquid chromatographic method for simultaneous determination of five residues of five herbicides in soybean. Methods: After the sample was extracted with methanol and cleaned up with C18 solid phase extraction cartridge, the sample was separated by high performance liquid chromatography. The detection wavelength was 230 nm. Results: The linearity of the five herbicides (propoxur, bentazon, fomesafen, quizalofop, 2,4-D) was good in the range of 0.5-10.0 mg / L and the recovery was 77.9% The relative standard deviations were 1.24% -6.50%. The limit of quantification was 0.05-0.08 mg / kg, which met the requirements of the state for the above residues of herbicides. Conclusion: This method is simple, accurate and reliable, providing a reference for quality control.