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目的探讨攀枝花地区2009年10～12月婴幼儿轮状病毒腹泻的分子流行病学特征及临床特点。方法采用胶体金免疫层析法、逆转录-聚合酶链法(RT-PCR)对收集的婴幼儿腹泻标本270份进行轮状病毒检测及A组轮状病毒常见的G、P基因分型研究。结果 270份腹泻标本轮状病毒阳性率为38.6%;6月～2岁年龄组的阳性率为45.4%(89/196),2～5岁年龄组的阳性率为20.3%(15/74),两组对比差异有统计学意义(P﹤0.005);104例被检测的A组轮状病毒腹泻粪便标本中G分型以G2为主,P分型以P4为主。结论轮状病毒是攀枝花地区婴幼儿腹泻的主要病原,G2型和P4型为2009年的优势毒株。该病毒引起的小儿腹泻主要发生在秋冬季,6月～2岁婴幼儿是轮状病毒的易感人群。
Objective To investigate the molecular epidemiology and clinical features of infantile rotavirus diarrhea in Panzhihua from October to December in 2009. Methods 270 samples of infantile diarrhea were detected by colloidal gold immunochromatography and reverse transcription-polymerase chain reaction (RT-PCR) for rotavirus and G and P genotyping of group A rotavirus . Results The positive rate of rotavirus in 270 samples of diarrhea was 38.6%. The positive rate was 45.4% (89/196) in June ~ 2 years old group and 20.3% (15/74) in 2 ~ 5 year old group , The difference between the two groups was statistically significant (P <0.005). The G genotypes of 104 cases of A group rotavirus diarrhea stool specimens were mainly G2, and the P type was mainly P4. Conclusions Rotavirus is the main cause of diarrhea in infants and young children in Panzhihua, while G2 and P4 are the dominant strains in 2009. The virus caused by diarrhea in children mainly occurs in autumn and winter, June to 2-year-old infants are rotavirus susceptible population.