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目的探讨胰岛素早期强化治疗对发病非肥胖糖尿病(NOD)小鼠胰岛炎及对胰岛细胞凋亡与再生的影响。方法选取近期发病NOD小鼠12只,随机平均分为A、B两组,A组给予胰岛素早期强化治疗,B组为发病未治疗组。同时选取同周龄未发病小鼠6只为C组。B和C组分别于分组当日即开始予PBS缓冲液对照治疗。B组于20 d处死,A和C组于60 d处死。HE染色观察各组小鼠胰岛炎性积分,免疫组化观察小鼠胰岛bax、bcl-2蛋白的表达。结果 A组小鼠糖尿病症状得到明显控制,其胰岛炎性积分显著低于B组(P<0.05),与C组相比差异无统计学意义(P>0.05)。A组bax表达低于B组,但差异无统计学意义(P>0.05),与C组相比差异有统计学意义(P<0.05)。A组bcl-2表达显著高于B组和C组(P<0.05)。结论胰岛素早期强化治疗能够减轻胰岛炎症,减少细胞凋亡,促进胰岛细胞再生。
Objective To investigate the effect of early intensive insulin treatment on insulitis and apoptosis and regeneration of islet cells in non-obese diabetic (NOD) mice. Methods Twelve NOD-prone mice were randomly divided into A and B groups randomly. A group received early intensive insulin treatment and B group was untreated group. At the same time, 6 mice of the same age were selected as C group. B and C groups were treated with PBS buffer control on the day of grouping respectively. Group B was sacrificed at 20 days and groups A and C were sacrificed at 60 days. The inflammatory integral of islets in each group was observed by HE staining, and the expression of bax and bcl-2 protein was observed by immunohistochemistry. Results The symptoms of diabetic mice in group A were significantly controlled, and their insulitis scores were significantly lower than those in group B (P <0.05). There was no significant difference between group A and group C (P> 0.05). The expression of bax in group A was lower than that in group B, but the difference was not statistically significant (P> 0.05). The difference was statistically significant compared with group C (P <0.05). The expression of bcl-2 in group A was significantly higher than those in group B and C (P <0.05). Conclusions Early intensive insulin treatment can reduce islet inflammation, reduce apoptosis and promote islet cell regeneration.