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[目的]建立国产巴西人参药材的质量标准。[方法]采用TLC法对国产巴西人参药材进行定性鉴别;用HPLC法对齐墩果酸进行定量研究。KromasilC-18250×4.6mm,5μm100A色谱柱,甲醇-水-乙酸(80︰20︰0.6)为流动相,流速1.0ml.min-1,检测波长220nm;柱温30℃;进样量20μl。[结果]在TLC色谱中能检出国产巴西人参。齐墩果酸在0.60～4.80μg范围内线性关系良好(r=0.9995),平均回收率为99.70%,RSD为1.61%(n=6)。测得国产巴西人参药材1.0g水解后的齐墩果酸含量范围为3.4484～3.5157mg。[结论]该方法简便、准确、快速、灵敏度高、重现性好,适用于巴西人参药材的质量控制。
[Objective] To establish the quality standard of domestic ginseng medicinal materials in Brazil. [Method] TLC method was used to qualitatively identify domestic ginseng medicinal materials. Quantitative analysis of oleanolic acid by HPLC method was carried out. Kromasil C-18250 × 4.6mm, 5μm 100A column, methanol-water-acetic acid (80:20︰0.6) as mobile phase, flow rate 1.0ml.min-1, detection wavelength 220nm; [Result] The domestic ginseng can be detected in TLC. Oleanolic acid had a good linear relationship (r = 0.9995) in the range of 0.60 ~ 4.80μg with an average recovery of 99.70% and RSD of 1.61% (n = 6). The results showed that the content of oleanolic acid in 1.0 g of domestic ginseng after hydrolysis was 3.4484 ~ 3.5157 mg. [Conclusion] The method is simple, accurate, rapid, sensitive and reproducible. It is suitable for the quality control of ginseng in Brazil.