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目的 探讨在日本血吸虫中间宿主湖北钉螺体内叶巢外睾吸虫和日本血吸虫的对抗性。 方法 通过叶巢外睾吸虫和日本血吸虫对湖北钉螺的双重感染 ,计算血吸虫的感染率和尾蚴逸出量 ,常规石蜡切片观察钉螺组织学变化。 结果 钉螺在感染血吸虫 37d后再感染外睾吸虫 ,经一定时间后检查发现 ,血吸虫的感染率为 5 2 9% ,显著低于同时单独感染日本血吸虫对照组的感染率 (75 9% ) ;先感染叶巢外睾吸虫 ,经 10、 32、6 0、 10 0和 12 0d不同的时间间隔后再感染日本血吸虫的钉螺 ,血吸虫感染率分别为 6 4 %、 6 6 7%、 6 5 2 %、5 6 4 %和 5 7 1% ,而单独感染日本血吸虫对照组钉螺血吸虫感染率为 90 5 % ,经统计检验 ,各双重感染实验组血吸虫与对照组的感染率间差异具有显著或非常显著性意义 (P <0 0 5或P <0 0 1)。对各试验组及对照组钉螺逸出尾蚴试验发现 ,试验组钉螺血吸虫尾蚴逸出量均显著低于对照组的逸出量。组织学观察发现各双重感染组钉螺消化腺萎缩 ,消化腺盲囊间隙只有少量血吸虫子胞蚴 ,血吸虫子胞蚴皱缩、变形及不规则 ,且子胞蚴中只含稀疏的尾蚴胚球 ,有的子胞蚴中已无胚球 ;而单独感染血吸虫的对照组中血吸虫均发育到成熟的子胞蚴或尾蚴。 结论 钉螺体内叶巢外睾吸虫和日本血吸虫之间存
Objective To investigate the antagonism of Schistosoma japonicum and Schistosoma japonicum in the Japanese snail schistosome intermediate host Hubei snail. Methods Infection of Schistosoma japonicum and Schistosoma japonicum on the infection of snails in Hubei Province, the infection rate of schistosoma japonicum and the release of cercariae were calculated, and histological changes of snails were observed with conventional paraffin sections. Results Inoculated with Schistosoma japonicum for 37 days, snails infected with Schistosoma japonicum again. After a certain period of time, the prevalence of schistosoma infection was 52.9%, which was significantly lower than that of the control group (75.9%) infected with Schistosoma japonicum alone. Infected with Schistosoma japonicum, the infection rate of Schistosoma japonicum was 64%, 66.7% and 65.2% at different time intervals of 10, 32, 60, 100 and 120d, respectively. , 54.4% and 57.1%, respectively, while the infection rate of S. japonicum alone was 90.5% in the control group. The statistical analysis showed that there was significant or significant difference in the infection rates between the schistosoma infection control group and the control group Sexual significance (P <0 0 5 or P <0 0 1). Tests on each test group and the control group snail escaped cercariae test found that the test group snail sperm cercariae discharge were significantly lower than the control group escape. Histological observation found that the snail digestive gland atrophy in the double infection group, digestive gland sac space only a small amount of schistosomes, Schistosome cercaria shrinkage, deformation and irregular, and only contain sparse metaplasia cercaria blastocyst, Some of the sub-cell larvae have no germ; and schistosoma schistosomiasis alone control group were developed to mature sporozoites or cercariae. Conclusion There is a gap between Schistosoma japonicum and Schistosoma japonicum in the snail