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目的:探讨当归散的不同化学组分对低子宫容受性小鼠受孕率的影响差异,筛选改善控制性超促排卵(COH)所致受孕率降低的最佳化学组分配方。方法:采用溶剂法对当归散中化学组分进行系统分离,分离出4个不同极性化学成分群,即石油醚提取物(组分A)、乙酸乙酯提取物(组分B)、乙醇提取物(组分C)和水提取物(组分D),并采用正交设计法配制出化学组分配方。采用“曲普瑞林+尿促性素(HMG)+绒促性素(HCG)”长周期超促排卵法制备小鼠低子宫容受性模型,造模同时预防性给予各化学组分配方,共9d。排卵当日合笼,于孕8d剖取子宫,清点胚泡。结果:当归散中组分A、B、D对受孕率具显著性影响,组分B、D使用可提高小鼠受孕率,而组分A则使受孕率降低。结论:当归散可以改善促排卵引起的子宫容受性降低,其中BCD化学组分配方为最优配方。
OBJECTIVE: To investigate the effects of different chemical components of Angelica sinensis on the pregnancy rate of mice with low uterine receptivity, and to screen the best prescription for reducing the pregnancy rate caused by controlled ovarian hyperstimulation (COH). Methods: The chemical constituents of Angelica sinensis were systematically isolated by solvent method. Four groups of chemical constituents with different polarities were isolated, namely petroleum ether extract (component A), ethyl acetate extract (component B), ethanol Extract (component C) and water extract (component D), and use orthogonal design to formulate chemical components formula. The mice model of low uterine receptivity was prepared by “long-term hyperstimulation” with “triptorelin + HMG + HCG”, and the model was given prophylactically to each group Distribution side, a total of 9d. Ovulation day cage, uterus in pregnancy 8d, inventory blastocyst. Results: Components A, B and D of Angelica sinensis had a significant effect on the pregnancy rate. The use of components B and D increased the pregnancy rate of mice, while the component A reduced the rate of pregnancy. Conclusion: Angelica powder can improve the reduction of uterine tolerance caused by ovulation induction, and the formula of BCD is the optimal formula.