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【目的】从分子水平研究嵩草品种资源之间的遗传多样性,为综合评价青藏高原嵩草种质资源提供依据。【方法】用筛选出的4对E+3/M+3引物对11份嵩草基因组DNA进行AFLP扩增。【结果】共得到164条清晰可辨条带,多态性条带154条,多态性位点百分率为93.96%,嵩草平均Nei’s基因多样性指数为0.2430,Shannon’s多样性指数为0.4012,表明嵩草种质间存在丰富的遗传多样性。通过UPGMA聚类分析,将11个嵩草居群划分为5类。【结论】嵩草的11个自然居群存在丰富遗传多样性,嵩草居群的遗传相似系数与海拔之间没有相关性,嵩草居群的生境的异质性影响遗传分化。
【Objective】 The genetic diversity among Kobresia species was studied at the molecular level to provide basis for comprehensive evaluation of Kobresia humilis resources in the Qinghai-Tibet Plateau. 【Method】 AFLP amplification was performed on 11 Kobresia genomic DNA using 4 pairs of E + 3 / M + 3 primers. 【Result】 A total of 164 clear bands and 154 polymorphic bands were obtained. The percentage of polymorphic loci was 93.96%. The average Nei’s gene diversity index was 0.2430 and the Shannon’s diversity index was 0.4012 Kobresia germplasm is rich in genetic diversity. By UPGMA cluster analysis, 11 Kobresia populations were divided into five categories. 【Conclusion】 There are abundant genetic diversity in 11 natural populations of Kobresia pygmaea. There is no correlation between the genetic similarity coefficient of Kobresia pygmaea population and altitude. The heterogeneity of the habitats of Kobresia pygmaea population affects genetic differentiation.