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目的探讨胞质线粒体的氧化磷酸化(OXPHOS)活性或线粒体DNA复制在卵子成熟、受精和胚胎发育过程中的作用。方法通过在小鼠体外成熟培养液中引入不同浓度的羰基氰4-(三氟甲氧基)苯腙(FCCP,10 nmol/L和100nmol/L)或2’,3’-双脱氧胞苷(ddC,10μmol/L和100μmol/L),抑制线粒体OXPHOS活性或线粒体DNA复制,统计分析各组卵子的体外生发泡破裂(GVBD)率、核成熟率、受精率及囊胚形成率,以分析线粒体功能抑制对卵子成熟、受精和胚胎发育的影响。结果线粒体OXPHOS活性和DNA复制功能在卵子和胚胎中所发挥的作用并不完全相同。FCCP抑制线粒体OXPHOS活性可显著降低卵子的核成熟率和囊胚形成率;但对卵子的GVBD的发生率和受精率无显著影响。而ddC抑制线粒体DNA复制不影响卵子的体外成熟和受精,但可显著抑制囊胚的形成。结论 OXPHOS活性主要影响卵子成熟及胚胎发育;线粒体DNA复制则主要影响胚胎发育;而线粒体功能抑制不影响卵子的成熟启动和体外受精。
Objective To investigate the role of mitochondrial oxidative phosphorylation (OXPHOS) activity or mitochondrial DNA replication during egg maturation, fertilization and embryonic development. Methods Different concentrations of 4- (trifluoromethoxy) phenylhydrazone (FCCP, 10 nmol / L and 100 nmol / L) or 2 ’, 3’-dideoxycytidine (ddC, 10μmol / L and 100μmol / L), inhibiting the activity of mitochondrial OXPHOS or mitochondrial DNA replication. The incidence of germinal vesicle rupture (GVBD), nuclear maturation, fertilization rate and blastocyst formation rate were analyzed statistically Mitochondrial function inhibits egg maturation, fertilization and embryonic development. Results The roles of mitochondrial OXPHOS activity and DNA replication in egg and embryo are not exactly the same. FCCP inhibition of mitochondrial OXPHOS activity can significantly reduce the egg nuclear maturation and blastocyst formation rate; but the incidence of egg and GVBD no significant effect on fertilization rate. While ddC inhibition of mitochondrial DNA replication does not affect in vitro egg maturation and fertilization, but can significantly inhibit the formation of blastocysts. Conclusion OXPHOS activity mainly affects egg maturation and embryo development. Mitochondrial DNA replication mainly affects embryonic development. However, mitochondrial function inhibition does not affect egg initiation and in vitro fertilization.