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目的观察葱白提取物及其不同制剂对急性心肌缺血兔血清一氧化氮(NO)和肌钙蛋白T(TnT)的影响,探讨葱白提取物纳米乳口腔喷雾剂治疗急性心肌缺血的作用机制。方法选用体重为2.5~3.0 kg的健康雄性日本大耳白兔48只,随机分为假手术组、空白模型组、硝酸甘油组、葱白提取物组、葱白纳米乳组和葱白纳米乳原位凝胶组,每组8只。制备急性心肌缺血模型,术后及时给药,假手术组和空白模型组舌下给予同体积的生理盐水0.5 ml,各治疗组分别给予相应的药物0.5 ml。给药后观察3 h,打开腹腔,腹主动脉取血,测定血清NO、TnT的含量。结果葱白提取物组、葱白纳米乳组、葱白纳米乳原位凝胶组血清中NO水平均显著升高(P<0.05),血清中TnT水平均明显降低(P<0.05);硝酸甘油组、葱白提取物组、葱白纳米乳组、葱白纳米乳原位凝胶组之间比较,差异无统计学意义。结论葱白提取物及其纳米乳和纳米乳原位凝胶均能促进血管舒张因子的分泌而改善心肌缺血,但是不同制剂之间差异无统计学意义,其原因还有待进一步深入研究。
Objective To observe the effects of extract of scutellaria baicalensis and their different preparations on the levels of serum nitric oxide (NO) and troponin T (TnT) in rabbits with acute myocardial ischemia, and to explore the mechanism of action of the extract of scutellaria barbae nanoemulsion oral spray on acute myocardial ischemia . Methods Forty eight healthy male Japanese white rabbits weighing 2.5-3.0 kg were randomly divided into three groups: sham operation group, blank model group, nitroglycerin group, Allium extract group, Alliums group, Glue group, each group of 8. The model of acute myocardial ischemia was prepared and administrated promptly after operation. The same volume of saline 0.5 ml was given subcutaneously to the sham operation group and the blank model group, and 0.5 ml of the corresponding drug was given to each treatment group. Observed after 3 h administration, open the abdominal cavity, abdominal aorta blood, serum NO, TnT levels. Results The levels of NO in serum of Allium scopariae extract group, Allied albumin group and Allied albumin group were significantly increased (P <0.05), while the levels of TnT in serum were significantly decreased (P <0.05) There was no significant difference between the groups of the light blue extract, the light blue nano-emulsion and the light blue nano-emulsion in situ gel. Conclusion The extract of scutellaria baicalensis Georgi and its in situ gels of nanoemulsion and nanoemulsion both promote the secretion of vasodilators and improve the myocardial ischemia, but there is no significant difference between different preparations. The reason remains to be further studied.