论文部分内容阅读
实验通过测定草鱼的24h半致死浓度,鳃的细胞结构以及sod和hsp90的表达模式研究了草鱼在组织学和分子生物学水平上对高浓度氨氮暴露的响应。经过半致死实验确定的氨氮24h LC50为243 mg/L试验中草鱼被置于5个浓度的处理组中(50、72、104、151、220 mg/L),之后取鳃组织进行组织切片分析,取肝脏、肠和鳃来测定sod和hsp90的表达情况。经过高浓度的氨氮暴露处理,鳃组织的细胞排列和结构产生了明显的变化,并且sod和hsp90的表达受到了显著的上调。这些结果表明,高浓度的氨氮能够损害鳃部的细胞结构并且诱导应激蛋白的表达。这个结果同样显示出,sod和hsp90可以作为评估草鱼氨氮暴露水平的良好指标。
The response of grass carp to high concentrations of ammonia-nitrogen exposure at the histological and molecular biology level was studied by measuring the lethal concentration of 24 hours, the cellular structure of gills and the expression patterns of sod and hsp90. The half-lethality determination of ammonia nitrogen 24h LC50 243 mg / L test was placed in five concentrations of grass carp treatment group (50,72,104,151,220 mg / L), gill tissue taken after the tissue section analysis , Take the liver, gut and gut to determine sod and hsp90 expression. After high concentration of ammonia exposure, the arrangement and structure of cells in gill tissue changed obviously, and the expressions of sod and hsp90 were significantly up-regulated. These results indicate that high concentrations of ammonia nitrogen can damage the cellular structure of the gills and induce the expression of stress proteins. This result also shows that sod and hsp90 can be used as good indicators to assess the level of ammonia nitrogen exposure in grass carp.