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目的探讨细胞外信号调节激酶(ERK)信号途径在五味子多糖对人宫颈癌Hela细胞凋亡和侵袭过程中的作用。方法将Hela细胞分为对照组,五味子多糖低剂量组(2.5mg·ml-1)、五味子多糖中剂量组(5mg·ml-1)和五味子多糖高剂量组(10mg·ml-1)等四组。用不同剂量的五味子多糖处理Hela细胞24 h后,MTT法检测细胞存活率,流式细胞仪Annexin V-FITC检测细胞凋亡,transwell法检测Hela细胞的侵袭情况,Western blot检测Hela细胞中凋亡相关蛋白及p-ERK和ERK等蛋白的表达。结果与对照组比较,五味子多糖高、中、低剂量组均能不同程度抑制Hela细胞的生长,诱导细胞凋亡,抑制肿瘤细胞的侵袭活性,降低p-ERK和Bcl-2蛋白的表达,增加Bax蛋白表达,并具有一定的剂量依赖关系。结论五味子多糖能够抑制人宫颈癌Hela细胞的增殖和侵袭,促进细胞的凋亡,ERK信号途径可能参与了五味子多糖对肿瘤细胞的抑制作用,为进一步研究五味子多糖抗宫颈癌打下了基础。
Objective To investigate the role of extracellular signal-regulated kinase (ERK) signaling in the apoptosis and invasion of human cervical cancer Hela cells. Methods Hela cells were divided into control group, low-dose group of Schisandra chinensis polysaccharides (2.5mg · ml-1), Schisandra polysaccharide middle-dose group (5mg · ml-1) and Schisandra chinensis polysaccharide high dose group (10mg · ml- group. Hela cells were treated with different doses of Schisandra chinensis polysaccharides for 24 h, the cell viability was detected by MTT assay, the apoptosis was detected by flow cytometry Annexin V-FITC, the invasion of Hela cells by transwell assay, the apoptosis of Hela cells by Western blot Related proteins and p-ERK and ERK protein expression. Results Compared with the control group, the Schisandra chinensis polysaccharide could inhibit the growth of Hela cells to a certain extent, induce the apoptosis, inhibit the invasion activity of tumor cells, reduce the expression of p-ERK and Bcl-2 protein, and increase Bax protein expression, and has a certain dose-dependent relationship. Conclusion Schisandra polysaccharide can inhibit the proliferation and invasion of human cervical cancer Hela cells and promote cell apoptosis. ERK signaling pathway may be involved in the inhibitory effect of Schisandra polysaccharides on tumor cells, which laid the foundation for further study of Schisandra polysaccharide against cervical cancer.