,Protein-protein interaction analysis in crude bacterial lysates using combinational method of 19F s

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Dear Editor,Protein-protein interactions (PPI) are essential for a variety of cellular functions.Many PPI analyses were conducted in vitro,using purified proteins.In this report,the unnatural amino acid tfmF was site-specifically incorporated into several different sites of two Phox-Bem1 (PB1) domains from two mitogen activated protein kinases (MEKK3 and/ or MEK5) in the E.coli cells.
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