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在水温分别为20(常温对照组)、25、30℃条件下,采用RT-PCR方法,研究性腺成熟期雄性黄颡鱼组织中P450芳香化酶(P450aromA和P450aromB)的mRNA表达水平,同时测定性腺成熟系数(GSI)、肝重指数(HSI)、肥满度(CF)和血浆睾酮(T),雌二醇(E2)含量。结果表明,P450aromA在性腺发育成熟期雄性黄颡鱼心脏、肝、脾、胃、肾脏、精巢、鳃、脑、头肾、肠管组织中均无表达,P450aromB只在脑和肠中有表达,在脑中表达量高于肠,表达量随着水温升高而显著下降;各组实验鱼血浆T和E2含量与芳香化酶基因表达量存在相关关系。该实验结果表明,P450aromA可能与精子发生相关,可能不参与黄颡鱼精子排放过程;P450aromB在雄鱼脑中高度表达,可能参与性腺成熟期精子活力保持与排放过程,且应激温度越高,对性腺成熟期精子活力保持与排放过程影响越大。
The mRNA expression levels of P450aromA and P450aromB in gonadal mature male Catfish were studied by RT-PCR at the water temperature of 20 (room temperature control group) and 25, 30 ℃ respectively. Meanwhile, GSI, HSI, CF and T and E2 were measured. The results showed that P450aromA was not expressed in the heart, liver, spleen, stomach, kidney, testis, gill, brain, head kidney and intestine of male catfish during the period of gonadal development. P450aromB was only expressed in brain and intestine. The expression level in brain was higher than that in intestine, and the expression level decreased significantly with the increase of water temperature. There was a correlation between the content of T and E2 in plasma and the expression of aromatase gene in each group. The results showed that P450aromA may be related to spermatogenesis, may not be involved in the process of sperm discharge of yellow catfish; P450aromB is highly expressed in male brain, may be involved in sperm motility during gonadal maturation and discharge process, and the higher the temperature, The greater the effect on sperm motility maintenance and release during gonadal maturation.