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背景:雌激素对帕金森病(Parkinson’sDisease,PD)可能具有保护作用,这为PD保护因素的研究提供了一条新的线索。目的:探讨雌激素对帕金森病是否具有保护作用。设计:完全随机对照实验研究。地点和材料:本研究在华中科技大学同济医学院附属协和医院神经内科实验室进行,材料为Bab/c小鼠96只,雌性,由同济医科大学动物学部提供,体质量20～25g,平均(21.8+2.5)g。干预:将实验小鼠随机分为3大组:A组(正常雌性小鼠MPTP造模组);B组(去势后MPTP造模组);C组(MPTP造模后去势组)。其中B组又分为两组,给予生理盐水组和给予雌激素组;C组分为两组,给予生理盐水组和给予雌激素组。主要观察指标:利用免疫组化染色(ABC法)计数酪氨酸羟化酶(TH)的阳性细胞数,用TUNEL法观察每视野下凋亡细胞的数目,比较各组的差异。结果:A组比B组中给予生理盐水组和C组中给予生理盐水组的TH阳性细胞数目多,凋亡细胞数目少(t=1.92,P<0.05);B组中给予生理盐水组与C组中给予生理盐水组差异无显著性意义(t=1.03,P>0.05);B组中给予雌激素组比B组中给予生理盐水组;C组中给予雌激素组比C组中给予生理盐水组TH阳性细胞多,凋亡细胞数目少(t=1.97,P<0.05)。结论:雌激素对帕金森病具有保护作用和损伤修复作用,且与凋亡有关。
BACKGROUND: Estrogen may have a protective effect on Parkinson’s disease (PD), which provides a new clue for the study of PD protection. Objective: To investigate whether estrogen has a protective effect on Parkinson’s disease. Design: Complete randomized controlled experimental study. Location and Materials: The study was performed at the Neurology Laboratory of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology. The materials were 96 Bab / c mice and female, provided by Department of Zoology, Tongji Medical University. The average body weight was 20-25g. 21.8 + 2.5) g. Intervention: The experimental mice were randomly divided into three groups: group A (normal female mouse MPTP model group); group B (post castration MPTP model group); group C (MPTP modeling castration group). Group B was divided into two groups, given saline group and given estrogen group; C group was divided into two groups, given saline group and given estrogen group. MAIN OUTCOME MEASURES: The number of tyrosine hydroxylase (TH) positive cells was counted by immunohistochemical staining (ABC method). The number of apoptotic cells per field was observed by TUNEL method and the differences among groups were compared. Results: The numbers of TH positive cells in group A and group C were significantly higher than those in group B (P <0.05). In group B, the number of TH positive cells in group B There was no significant difference between group C and group C (t = 1.03, P> 0.05). Group B received estrogen and group B received saline; Group C received estrogen than group C In the saline group, there were more TH positive cells and fewer apoptotic cells (t = 1.97, P <0.05). Conclusion: Estrogen has a protective effect on Parkinson’s disease and repair injury, and is related to apoptosis.