将已建系的人体骨肉瘤细胞HOS-8603复苏后扩大培养,用Hanks溶液处理,在无菌条件下,分别接种在4周龄NC和BALB/C裸小鼠腰背部及臀部皮下组织,5×10~6细胞/只,经10d左右潜伏期后生长肿瘤。一般当肿瘤长到2～3cm时传代。自1989年10月7日至1990年9月27日,共传9代,总的传代成功率为36/36(100％)。并对移植性骨肉瘤裸小鼠进行了病理研究,巨检见肿瘤组织表面呈结节状, The established human osteosarcoma cells HOS-8603 after resuscitation expansion of culture, treatment with Hanks, under sterile conditions were inoculated 4 weeks old NC and BALB / C nude mice back and hips subcutaneous tissue, 5 × 10 ~ 6 cells / only, after 10d incubation period of tumor growth. Usually when the tumor grows to 2 ~ 3cm passage. From October 7, 1989 to September 27, 1990, a total of 9 generations of transmission, the total pass success rate of 36/36 (100%). And the pathological study of transplanted osteosarcoma nude mice, see the surface of tumor tissue showed nodular,