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利用焦锑酸钙沉淀和硝酸铅沉淀的电镜细胞化学方法,以室温生长的北海道黄杨植株为对照,研究了人工4℃低温胁迫过程中北海道黄杨(Euonymusjaponicus‘Cuzhi’)叶肉细胞Ca2+和Ca2+-ATPase的动态变化。在4℃低温胁迫的初期(3~12h),北海道黄杨叶肉细胞间隙和液泡内的Ca2+沉淀颗粒减少,而细胞质和细胞核内的Ca2+水平升高,但Ca2+-ATPase在细胞的分布几乎没有变化,主要分布在质膜和液泡膜上,有较高的活性;低温胁迫24h,细胞质和细胞核内增加的Ca2+开始回到细胞间隙和液泡中,Ca2+-ATPase在质膜和液泡膜上活性增强;在低温胁迫48~96h,细胞内的Ca2+又回到低温胁迫前的低水平,但Ca2+-ATPase在质膜和液泡膜上仍有很高的活性。叶肉细胞内Ca2+稳态平衡和Ca2+-ATPase的活性变化与植物的抗寒性存在一定的相关性。
Using electron microscopy and cytochemical method of calcium pyroantimonate precipitation and lead nitrate precipitation, we compared the contents of Ca2 + and Ca2 + -ATPase in the mesophyll cells of Euonymus japonicus’Cuzhi ’under artificial cold stress of 4 ℃ Dynamic changes. At the initial stage of low temperature stress (3 ~ 12h), Ca2 + precipitates in the cell gap and vacuole of Hypoxanthoxylum bungeanum decreased, while Ca2 + levels in the cytoplasm and nucleus increased, but the distribution of Ca2 + -ATPase in the cells remained almost unchanged. Mainly distributed in the plasma membrane and tonoplast, and had higher activity. At 24 hours after cold stress, the increased Ca2 + in the cytoplasm and nucleus began to return to the intercellular space and vacuoles, and the activity of Ca2 + -ATPase increased on the plasma membrane and tonoplast Low temperature stress 48 ~ 96h, intracellular Ca2 + back to the low level before low temperature stress, but Ca2 + -ATPase in the plasma membrane and tonoplast still have high activity. The change of Ca2 + homeostasis and Ca2 + -ATPase activity in mesophyll cells is related to the cold resistance of plants.