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采用静水生物试验法，人工添加氨氮并检测与南方鲶免疫相关的两种抗氧化酶及抗菌活力的变 化，进行氨氮对南方鲶免疫水平影响的试验。结果表明：在本试验剂量范围内，氨氮在一定浓度时对南方鲶 超氧化物歧化酶(SOD)、过氧化物酶(POD)以及抗菌活力均表现出诱导作用；在氨氮浓度高于30 mg/L 时 各组 SOD 及 POD 活力均出现显著高于正常水平(P＜0． 05) 的活力值，之后随时间延长活力逐渐下降；高浓 度氨氮水平(150 mg/L与120 mg/L)对抗菌活力主要表现为抑制作用。试验表明氨氮对南方鲶存在一定的 “阈值”，只有水体实际氨氮浓度超过这一作用“阈值”(30～60 mg/L 之间)时才会表现出诱导作用。
Hydrostatic tests were used to test the effects of ammonia nitrogen on the immune function of southern catfish by artificially adding ammonia nitrogen and detecting the changes of two anti-oxidative enzymes and antibacterial activities related to the immunity of southern catfish. The results showed that ammonia nitrogen showed an induction effect on the activities of superoxide dismutase (SOD), peroxidase (POD) and antibacterial activity in the southern catfish at a certain concentration within the range of the test dose. Under the conditions of ammonia concentration above 30 mg / L, the activity of SOD and POD in each group were significantly higher than the normal level (P <0.05), and then decreased with time. The high concentration of ammonia nitrogen (150 mg / L and 120 mg / L) Antibacterial activity mainly for the inhibition. Tests showed that ammonia nitrogen had a certain “threshold” on the southern catfish, and only showed an induction effect if the actual ammonia concentration in water exceeded the “threshold” (30-60 mg / L).