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目的：报道了人体内艾司唑仑及其主要代谢物４′羟基艾司唑仑的电喷雾离子阱质谱鉴定法。方法：采用固相萃取或液－液萃取从生物基质中提取待测物，然后将其直接导入ＥＳＩ离子源进行质谱分析。结果：选择ｍ／ｚ２９５和ｍ／ｚ３１１为母离子，分别进行ＭＳ２和ＭＳ３级质谱分析，以它们生成的子离子（ＭＳ２：ｍ／ｚ２６７，ＭＳ３：ｍ／ｚ２４０和２０５）及其主要代谢物（ＭＳ２：ｍ／ｚ２８３，ＭＳ３：ｍ／ｚ２５６和２２１）来鉴定母体药物。结论：本法专属性强，灵敏度高（母体药物的ＬＯＤ为０５ｎｇ·ｍＬ－１），可作为法医毒物分析的一种方法。
OBJECTIVE: The electrospray ion trap mass spectrometry method for the determination of estazolam and its major metabolite 4’-hydroxy-estazolam in human is reported. Methods: The analytes were extracted from the biological matrix by solid-phase extraction or liquid-liquid extraction and then directly introduced into the ESI ion source for mass spectrometry analysis. Results: Mass spectra of m / z295 and m / z311 were selected as MS1 and MS3, respectively. Based on the mass spectra of their daughter ions (MS2: m / z267, MS3: m / z240 and 205) and their major metabolites MS2: m / z 283, MS3: m / z 256 and 221) to identify the parent drug. Conclusion: This method is highly specific and sensitive (LOD of parent drug is 0.5 ng · mL-1), which can be used as a method of forensic toxicological analysis.