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目的测定不同采收期紫丁香叶中原儿茶酸、原儿茶醛、酪醇的含量。方法采用RP-HPLC直接测定。YWG-C18色谱柱(4.6mm×250mm,10μm);流动相为甲醇-0.125%冰醋酸溶液(10︰90),流速1.0mL·min-1;检测波长275nm。结果原儿茶酸在0.08～0.56μg内呈良好线性,回归方程为Y=83995X-10368,r=0.9995;原儿茶醛在0.01～0.07μg内呈良好线性,回归方程为Y=111202X-35323,r=0.9998;酪醇在0.42～2.94μg内呈良好线性,回归方程为Y=139922X-32003,r=0.9996。紫丁香叶中原儿茶酸的含量10月份最高,原儿茶醛和酪醇的含量4月份最高。结论该方法简便、快速、准确可靠,适用于紫丁香叶中原儿茶酸、原儿茶醛、酪醇多种成分的同时测定;适用于紫丁香叶的质量控制。结果表明紫丁香叶以10月份或4～5月份采收为宜。
Objective To determine the content of protocatechuic acid, protocatechuic aldehyde and tyrosol in different clove leaves. Methods were determined directly by RP-HPLC. YWG-C18 column (4.6mm×250mm, 10μm); mobile phase methanol-0.125% glacial acetic acid solution (10..90), flow rate 1.0mL·min-1; detection wavelength 275nm. Results Protocatechuic acid showed good linearity in the range of 0.08-0.56 μg. The regression equation was Y=83995X-10368, r=0.9995. The protocatechuic aldehyde was linear in the range of 0.01-0.07 μg. The regression equation was Y=111202X-35323. , r = 0.9998; tyrosol was linear in 0.42 ~ 2.94μg, the regression equation was Y = 139922X-32003, r = 0.9996. The content of protocatechuic acid in lilac leaves was the highest in October, and the content of protocatechuic aldehydes and tyrosol was the highest in April. Conclusion The method is simple, rapid, accurate and reliable. It is suitable for the simultaneous determination of various components of protocatechuic acid, protocatechuic aldehyde and tyrosol in Syringa. It is suitable for the quality control of Syringa. The results showed that the lilac leaves were harvested in October or April to May.