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目的建立黑龙江产万寿菊花醇提物的指纹图谱并定性分析共有峰,为万寿菊花的质量控制提供参考。方法采用HPLC分离UV检测建立色谱指纹图谱,Zorbax Eclipse XDB C18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.2%磷酸水溶液梯度洗脱,体积流量1 mL/min,柱温25℃,检测波长254 nm;用LC-MS定性共有峰,电喷雾离子源(ESI),负离子模式扫描,雾化器压力241.325 kPa,干燥器温度350℃,干燥气体积流量10 L/min,扫描范围:m/z 50～1 000。结果万寿菊花醇提物的指纹图谱有8个共有峰,并具有较好的精密度、重复性和稳定性,其中5个共有峰所对应的化学物质为万寿菊素、异槲皮素、异槲皮素苷和槲皮万寿菊素及其苷。结论建立的万寿菊花醇提物的指纹图谱,为万寿菊花的质量控制提供参考。
OBJECTIVE To establish the fingerprint of ethanol extract of marigold flower from Heilongjiang and to qualitatively analyze the common peaks and provide a reference for the quality control of marigold flower. Methods Chromatographic fingerprints were prepared by HPLC. The chromatographic fingerprints were developed on a Zorbax Eclipse XDB C18 column (250 mm × 4.6 mm, 5 μm) with a gradient of acetonitrile-0.2% phosphoric acid. The volume flow rate was 1 mL / min and the column temperature was 25 ℃. Wavelength 254 nm; qualitative peak shared by LC-MS, electrospray ionization source (ESI), negative ion mode scanning, atomizer pressure 241.325 kPa, dryer temperature 350 ℃, drying gas volume flow 10 L / min, scanning range: m / z 50 ~ 1 000. Results The fingerprints of marigold flower alcohol extract had 8 common peaks, and had good precision, repeatability and stability. Among them, the chemical components corresponding to 5 common peaks were marigoldin, isoquercetin, Quercetin glycosides and quercetin marigoldine and its glycosides. Conclusion The fingerprint of the marigold flower alcohol extract provides a reference for the quality control of marigold flower.