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以橘红色花菜薹突变体11A-47与黄色花菜薹联记特选34号甜菜心杂交获得的F1,F2及BC1、BC1′群体为试材。将6个世代的种子经4℃低温春化处理15d后调查子叶颜色,研究菜薹橘红色花的遗传规律;同时,采用与大白菜橘红心球色基因紧密连锁的分子标记对控制菜薹橘红色花的基因进行分析,鉴定菜薹橘红色花与大白菜橘红心球色基因or之间的关系。结果表明,橘红色花菜薹11A-47与黄色花菜薹杂交F2群体中,橘红色子叶与绿色子叶的分离比例符合1∶3,χ2=1.9389<χ20.05=3.841;BC1′群体中,橘红色子叶与绿色子叶的分离比例符合1∶1,χ2=1.3697<χ20.05=3.841。说明菜薹的橘红色花为质量性状,由1对隐性等位基因控制。分子标记结果表明,控制菜薹橘红色花的基因与控制大白菜橘红心球色的基因可能不同。
The F1, F2 and BC1, BC1 ’populations obtained from the cross of 11A - 47 orange flower cauliflower mutant and the yellow cauliflower sedge selection 34 sugar beet heart were used as materials. The seeds of 6 generations were treated with vernalization at 4 ℃ for 15 days and the color of cotyledons was investigated to study the genetic law of the orange flower of Campanulaceae. At the same time, the molecular markers closely linked to the orange globin gene of Chinese cabbage The gene of red flower was analyzed to identify the relationship between the red flower of Chinese flowering cabbage and the orange heart color gene of Chinese cabbage. The results showed that the segregation ratio of orange-red cotyledons and green cotyledons was 1: 3 and χ2 = 1.9389 <χ20.05 = 3.841. Among BC1 ’populations, the ratio of orange-red Cotyledons and green cotyledons separation ratio in line with 1: 1, χ2 = 1.3697 <χ20.05 = 3.841. Description bluebell flower quality of the flower traits, by a pair of recessive alleles control. The results of molecular markers showed that the genes controlling the orange-red flower of the Chinese cabbage may be different from the genes controlling the orange-red heart of the Chinese cabbage.