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以玉米优良自交系为材料, 利用芽尖分生组织诱导胚状体和丛生芽, 建立起一种快速有效且取材不受季节限制的玉米丛生芽诱导体系. 以丛生芽组织块为受体, 用基因枪将从拟南芥(Arabidopsis thaliana)突变体中克隆的抗除草剂基因als (acetolac- tate synthase)导入玉米细胞, 经除草剂chlorsulfuron筛选得到抗性组织块并再生植株. PCR检测和Southern杂交表明, 部分再生植株转入了als基因. 除草剂喷施试验表明, 转基因植株及其子一代具有良好的抗除草剂特性. 建立了一种新的受基因型限制小的玉米离体培养及转基因受体系统, 能快速有效地获得大批转基因植株.
Inbred maize inbred line as material, using shoot apical meristem tissue to induce embryoid body and clustered bud, to establish a fast and effective and not seasonally restrained corn cluster shoot induction system. , The herbicide resistant gene als (acetolac-tate synthase) cloned from the mutant of Arabidopsis thaliana was introduced into maize cells with a gene gun and screened by the herbicide chlorsulfuron to obtain the resistant tissue mass and regenerate the plants. PCR detection and Southern hybridization showed that some of the regenerated plants were transformed into als gene.Experimental herbicide spraying showed that the transgenic plants and their offspring had good herbicide tolerance and established a new in vitro genotype-restricted corn in vitro culture And transgenic receptor system, can quickly and efficiently obtain a large number of transgenic plants.