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目的 研究Graves病 (GD)甲状腺细胞凋亡和凋亡相关蛋白Fas、可溶性Fas(sFas)及Fas配基 (Fas L)的表达特征 ,探讨Fas介导的细胞凋亡与GD发病机制之间的内在联系。方法甲状腺组织取自 7例GD手术患者和 3例意外死亡健康个体 ,采用原代甲状腺细胞 (TEC)培养技术、流式细胞术、ELISA及半定量RT PCR法检测TEC凋亡率及Fas、sFas和Fas L的表达情况。结果(1)GDTEC凋亡率为 (3.42± 0 .81) %,明显高于正常对照 (P <0 .0 1)。 (2 )GDTECFas表达率为 (8.3± 1.7) %,与正常对照相比差异无显著性。 (3)GDTEC培养上清液中sFas含量明显高于正常对照(P <0 .0 1)。 (4 )正常及GDTEC均表达Fas及sFasmRNA ,而Fas LmRNA仅在GDTEC表达。与正常对照相比 ,GDTECFasmRNA差异无显著性 ,sFasmRNA含量则明显升高 (P <0 .0 1)。结论GD患者甲状腺细胞存在细胞凋亡和Fas系统的异常表达 ,提示Fas介导的细胞凋亡参与GD的发病过程 ,sFas产生增加可能与甲状腺细胞增殖有关。
Objective To investigate the expression of apoptosis-related proteins Fas, soluble Fas (sFas) and Fas ligand (Fas L) in thyrocytes of Graves disease (GD) and to explore the relationship between Fas-mediated apoptosis and the pathogenesis of GD inner relationship. Methods Thyroid tissue was taken from 7 patients with GD and 3 healthy individuals died from accidental death. The TEC apoptosis rate and Fas, sFas were detected by flow cytometry, semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) And Fas L expression. Results (1) The apoptotic rate of GDTEC was (3.42 ± 0.81)%, which was significantly higher than that of the normal control (P <0.01). (2) GDTECFas expression rate was (8.3 ± 1.7)%, compared with the normal control, no significant difference. (3) The content of sFas in GDTEC culture supernatant was significantly higher than that of the normal control (P <0.01). (4) Both normal and GDTEC express Fas and sFas mRNA, while Fas LmRNA is only expressed in GDTEC. Compared with the normal control, there was no significant difference in GDTECFasmRNA and sFasmRNA levels (P <0.01). Conclusion The abnormal expression of Fas and Fas in thyroid cells in GD patients suggests that Fas-mediated apoptosis is involved in the pathogenesis of GD. The increased production of sFas may be related to the proliferation of thyroid cells.