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目的：探讨维甲酸在预防和治疗肿瘤中的作用机制，分离受维甲酸调控的靶基因。方法：采用ＡＰ－ＰＣＲ、测序、Ｎｏｒｔｈ－ｅｍ杂交及序列同源分析等方法。结果：首次发现ＡＴＲＭ可上调线粒体基因ＡＴＰ酶第六亚单位表达，这种表达上调开始于Ａ－ＴＲＡ诱导早期并持续整个诱导过程（７ｄ）。 Ｎｏｒｔｈｅｍ杂交结果证实 ＡＴＲＡ对线粒体基因 ＡＴＰ酶第六亚单位的上调作用。对线粒体基因组的转录调控区Ｄ－ｌｏｏｐ序列进行了初步分析发现，该转录调控区两处存在着与维甲酸受体反应元件核心序列高度一致的外翻重复序列（ｅｖｅｒｔｅｄ ｒｅｐｅａｔｅｄ ｓｅｏｐｎｃｅｓ），其间隔分别为 １２ｂｐ和 １３ｂｐ。结论：维甲酸受体与线粒体基因组维甲酸反应元件相互作用可能是其调控线粒体基因的表达。维甲酸除调控核基因外，亦可对线粒体基因进行直接调控。
Objective: To investigate the mechanism of action of retinoic acid in the prevention and treatment of tumors and isolate target genes regulated by retinoic acid. Methods: AP-PCR, sequencing, North-em hybridization and sequence homology analysis were used. Results: It was found for the first time that ATRM can up-regulate the expression of the sixth subunit of the mitochondrial gene ATPase, and this up-regulation begins at the early stage of A-TRA induction and continues throughout the induction process (7d). Northem hybridization confirmed the upregulation of ATRA on the sixth subunit of the mitochondrial gene ATPase. A preliminary analysis of the D-loop sequence of the transcriptional regulatory region of the mitochondrial genome revealed that there are two episodic repeats (everted repeated seopnces) in the transcriptional regulatory region that are highly consistent with the core sequence of the retinoid receptor response element. 12bp and 13bp. Conclusion: The interaction of retinoic acid receptor and mitochondrial genome retinoids may be related to its regulation of mitochondrial gene expression. In addition to regulating nuclear genes, retinoic acid can also directly regulate mitochondrial genes.