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目的 探讨慢性乙肝患者外周血单个核细胞 (PBMC)内HBV -DNA的出现与血清HBV -DNA浓度之间关系 .方法 应用荧光定量PCR技术检测 5 7例慢性乙肝患者血清和PBMC中HBV -DNA含量、对血清中不同的病毒浓度进行分组比较分析 .结果 ① 5 7例PBMC内HBV DNA总检测出率为 4 2 .1% (2 4 / 5 7) ,两者检测结果一致占 88.6 % .②根据血清内HBV -DNA浓度分成三组 ,三组PBMC内HBV -DNA浓度及阳性率比较p <0 .0 1,存在显著差异 ;③血清HBV -DNA阴性而PBMCHBV -DNA阳性只有 1例 (1.7% ) ,其浓度为 1.5 0× 10 8/L .结论 ①血清HBV -DNA浓度与PBMC内HBV -DNA的出现及HBV -DNA浓度存在明显相关性 .②临床上检测PMBC中HBV -DNA是对血清HBV -DNA的一个重要补充 ;③PBMC内HBV -DNA检测对观察病毒在非血清内的状态、间接反映肝细胞病毒复制情况以及进一步指导抗病毒治疗有一定意义
Objective To investigate the relationship between HBV-DNA and serum HBV-DNA concentration in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B. Methods The levels of HBV-DNA in serum and PBMC of 57 chronic hepatitis B patients were detected by real-time fluorescence quantitative PCR , Respectively.Results ① The detection rate of HBV DNA in 57 PBMCs was 42.1% (24/57), which was consistent with 88.6% of the detection results.② According to the serum HBV-DNA concentration divided into three groups, three groups of PBMC HBV-DNA concentration and positive rate compared p <0 01, there is a significant difference; ③ serum HBV-DNA negative and PBMCHBV-DNA positive only 1 (1.7 %), The concentration was 1.5 0 × 10 8 / L. Conclusion ① The serum HBV-DNA concentration and the presence of HBV-DNA in PBMC and HBV-DNA concentration there is a significant correlation between ② clinical detection of HBV-DNA in PMBC Serum HBV-DNA an important complement; ③ HBV DNA-PCR detection of PBMC to observe the virus in the non-serum state, indirectly reflect the replication of hepatocytes and further guidance of anti-viral therapy has some significance