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目的:建立冬虫夏草的红外指纹图谱检测方法,对冬虫夏草野生品、繁育品以及伪虫草(蝉花、虫草花、中华被毛孢菌丝粉、蝙蝠蛾拟青霉菌丝粉)进行红外测试。方法:根据红外平均图谱、一阶导数图谱、二阶导数图谱和标准偏差图谱,判断这3种虫草的一致性。结果:在3000~2800 cm-1、1800~1000 cm-1、1000~800 cm-1,30批冬虫夏草繁育品和21批野生品的红外光谱图谱的指纹图谱具有较好的一致性,相似度均大于0.90;与伪虫草不一致,相似度均小于0.90。结论:该方法具有良好的专属性、重复性、稳定性和精密度,可用于冬虫夏草红外指纹图谱分析。
OBJECTIVE: To develop an infrared fingerprint method for the determination of Cordyceps Sinensis (Cordyceps sinensis), and to conduct infrared tests on the wild Cordyceps sinensis wild products, breeding products and pseudo-Cordyceps sinensis (Cordyceps, Cordyceps sinensis, Chrysopa sinica, and Paecilomyces militaris). Methods: According to the infrared average spectrum, the first derivative spectrum, the second derivative spectrum and the standard deviation map, the consistency of the three kinds of Chinese caterpillar fungus was determined. Results: The fingerprints of Cordyceps sinensis cultivars and 21 batches of wild Cordyceps sinensis cultivars from 3000 to 2800 cm-1, from 1800 cm to 1000 cm-1 and from 1000 to 800 cm-1 had good consistency and similarities Are greater than 0.90; inconsistent with pseudo-Cordyceps, the similarity is less than 0.90. Conclusion: This method has good specificity, repeatability, stability and precision, which can be used for infrared fingerprint analysis of Cordyceps sinensis.