本文依据国际种子检验协会（ＩＳＩＡ）颁布的小麦、大麦品种鉴定的酸性聚丙烯酰胺凝胶电泳（ＡＰＡＧＥ）方法，利用国产仪器、药品对其做了适合性检验，并就凝胶浓度、交联度及强度做了调整。对千余份普通小麦的ＡＰＡＧＥ分析表明，改良后的方法效果稳定，具有谱带分辨率高、重现性好、胶强度适宜、易剥离、制成干板后谱带不扩散及操作简便、快速等特点。认为是一套适合我国试验器材和药剂等实际条件的麦醇溶蛋白分离方法，可以做为品质研究、品种鉴定的可靠分析手段。 In this paper, according to the method of acid polyacrylamide gel electrophoresis (APAGE), which was appraised by the International Association of Seed Testing (ISIA) for the wheat and barley variety, the suitability test was carried out by using domestic instruments and drugs. The gel concentration, Degree and intensity have been adjusted. APAGE analysis of more than one part of common wheat showed that the improved method has the advantages of stable effect, high spectral resolution, good reproducibility, suitable glue strength, easy peel, non-proliferation of the band after being made into a dry plate and easy operation, Fast and so on. It is considered as a set of gliadin separation methods suitable for the actual conditions of China’s test equipment and pharmaceuticals, and can be used as a reliable analytical method for quality research and variety identification.