采用离体长期培养的肿瘤细胞系进行细胞遗传学研究,是探讨肿瘤生物学特性的一个重要方面。迄今,通过对肿瘤细胞系或短期培养的肿瘤活检组织的研究,已经发现了30余种肿瘤有恒定的克隆性染色体改变,并且表明,某些染色体重排是人类原癌基因激活的机理之一。近年来,已有人在非显带水平对肺腺癌 The use of long-term in vitro cultured tumor cell lines for cytogenetics is an important aspect to explore the biological characteristics of tumors. To date, studies of tumor cell lines or short-term cultured tumor biopsies have found constant clonal chromosomal alterations in more than 30 tumors and suggest that certain chromosomal rearrangements are one of the mechanisms by which human proto-oncogenes are activated . In recent years, there have been non-visible levels of lung adenocarcinoma