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用免疫组化方法检测43例乳腺癌标本ER、PR受体，其中21例同时作C－erbB－2基因检测；用图文分析系统对染色结果进行友度值测定。结果，图像分析显示C—erbB－2、ER、PR各组免疫组化染色阳性与阴性之间的灰度值有显著性差异。说明灰度值测定可作为免疫组化结果辅助判断方法之一，该法客观精确地表达了染色深度，有助于弱阳性的判断。
Immunohistochemical method was used to detect ER and PR receptors in 43 breast cancer specimens. Among them, 21 cases were simultaneously tested for C-erbB-2 gene; the image was used to analyze the staining results for the determination of friendship values. As a result, image analysis showed a significant difference in the gray value between positive and negative immunohistochemical staining in each group of C-erbB-2, ER, and PR. It is shown that the measurement of gray value can be used as one of the methods of assisting in the determination of immunohistochemical results. This method accurately and accurately expresses the depth of staining, which contributes to the determination of weak positives.