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运用HE和Nissl染色、免疫组织化学法、透射电镜及扫描电镜，对60例6周至足月的人胚胎海马室管膜上皮变化进行了观察。发现胚胎发育过程中室管膜发生了剧烈变化。最早室管层神经上皮细胞为假复层柱状，随着未分化细胞向外迁徙，海马室管膜层神经上皮细胞迅速增殖，形成复层上皮。当室管膜层细胞停止迁徙时，室管膜开始向假复层柱状及单层柱状上皮转变。电镜观察，胚胎早期神经上皮细胞由未分化细胞构成；其特点是，细胞质内各种特化细胞器匮乏，但糖原丰富。15周左右未分化细胞开始向长突细胞及室管膜细胞分化。长突细胞电子密度高，底部有细长突起，表面有微绒毛，胞质内微丝丰富；而室管膜细胞电子密度低，底部无突起，但表面有丰富的纤毛。对长突细胞及免疫组化染色的GFAP阳性细胞进行形态和发育特征的比较，提示两者属同一类细胞。扫描电镜下，15周前室管膜表面微绒毛较多，以后纤毛逐步发育，大量密集纤毛布满于室管膜表面。此外，还能见到一类接触脑脊液神经元，这类神经元可为多极或双极，并有突起伸入室管膜上皮内。
The changes of ependymal epithelium in 60 hippocampal of human embryo from 6 weeks to full term were observed by HE and Nissl staining, immunohistochemistry, transmission electron microscopy and scanning electron microscopy. The ependymal membrane was found to undergo dramatic changes during embryonic development. The earliest layer of neural tube epithelial cells pseudostratified columnar column, with the undifferentiated cells migrate outward, the hippocampal neuronal epithelial cells rapidly proliferate, forming the stratified epithelium. When the ependymal cells stopped migrating, ependymal cells began to migrate to pseudostratified columnar and monolayer columnar epithelium. Electron microscopy showed that the embryonic early neuroepithelial cells were composed of undifferentiated cells, which were characterized by the lack of various specialized organelles in the cytoplasm but rich in glycogen. At about 15 weeks, undifferentiated cells began to differentiate into long blastocysts and ependymal cells. Processed cells with high electron density, slender protrusions at the bottom, microvilli on the surface, and abundant cytoplasmic microfilaments; while the ependymal cells have low electron density and no protrusions at the bottom, but the surface is rich in cilia. Comparison of morphology and development of GFAP positive cells stained with long promy cells and immunohistochemistry suggested that they belong to the same type of cells. Scanning electron microscopy, 15 weeks before the ependymal surface microvilli more, after the gradual development of cilia, a large number of dense cilia covered in the ependymal surface. In addition, we can see a class of cerebrospinal fluid contact neurons, such neurons can be multipolar or bipolar, and have protrusions protruding into the ependymal epithelium.