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根据环核苷酸理化性质及乳的特点,采用酶法从乳汁中提取环核苷酸。研究表明,该方法条件温和、工艺合理、操作方便。经放射免疫测定提取浓缩液浓度为cAMP20μmol·L-1,cGMP0.2μmol·L-1.
According to the physical and chemical properties of the cyclic nucleotides and the characteristics of milk, cyclic nucleotides were extracted from the milk by enzymatic method. The research shows that the method has the advantages of mild conditions, reasonable process and convenient operation. Concentration of the extract by radioimmunoassay was cAMP20μmol·L-1 and cGMP0.2μmol·L-1.