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目的探讨胎肝细胞输注（ＦＬＩ）对化疗后机体免疫细胞的影响。方法对瓦克癌肉瘤（Ｗ２５６）大鼠环磷酰胺化疗后，再进行胎肝细胞输注，观察其对外周血各型ＡＮＡＥ淋巴细胞百分率的影响，并测定免疫器官重量，观察对脾指数及胸腺指数的影响。结果化疗后，环磷酰胺组（ＣＴＸ组）ＡＮＡＥ淋巴细胞总阳性率和点样型百分率分别为５６．９０±９．２９，４２．３０±７．５３；环磷酰胺加胎肝细胞输注组（ＣＴＸ＋ＦＬＩ组）分别为５１．６０±８．１３，４０．３０±６．８５，与正常对照组（ＮＳ组）相比显著降低（Ｐ＜０．０５），ＣＴＸ组胸腺指数为０．５８±０．３７，与ＮＳ组相比亦显著降低（Ｐ＜０．０５）。胎肝细胞输注后，ＣＴＸ＋ＦＬＩ组ＡＮＡＥ点样型百分率为６０．８０±１１．４３，与单纯化疗者相比显著升高（Ｐ＜０．０５）；胸腺指数为１．０３±０．５０，与ＣＴＸ组相比亦显著升高。结论胎肝细胞输注对环磷酰胺化疗引起的外周血点样型ＡＮＡＥ淋巴细胞百分率下降及胸腺重量减轻有促进恢复作用。
Objective To investigate the effect of fetal liver cell transfusion (FLI) on immune cells after chemotherapy. METHODS: Chemotherapy with cyclophosphamide (W256) in rats with Wake Carcinosarcoma (W256) was followed by fetal liver cell transfusion. The effect of peripheral blood on the percentage of AEA lymphocytes in various types of peripheral blood was observed. The weight of immune organs was measured and the spleen index was observed. Thymus index effect. Results After chemotherapy, the total positive rate and spotty percentage of ANAE lymphocytes in the CTX group were 56.90±9.29, 42.30±7.53, respectively; Cyclophosphamide plus fetal liver cell transfusion The group (CTX + FLI group) was 51.60 ± 8.13, 40.30 ± 6.85, respectively, compared with the normal control group (NS group) significantly lower (P <0.05), CTX group thymus index was 0. 58±0.37, also significantly decreased compared with NS group (P<0.05). After fetal liver cell transfusion, the percentage of NAAE spotted pattern in CTX+FLI group was 60.80±11.43, which was significantly higher than that of chemotherapy alone (P<0.05). The thymus index was 1.03±0.50. , compared with the CTX group also significantly increased. Conclusion Fetal liver cell infusion can promote the recovery of peripheral blood spot-type AAE lymphocytes and weight reduction of thymus caused by cyclophosphamide chemotherapy.