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背景:鸡冠花黄酮类化合物是一种植物性雌激素,具有多种生理功能,且无毒副作用,对骨质疏松的治疗和预防都具有良好干预效果。目的:探讨鸡冠花黄酮化合物对糖尿病大鼠骨形成蛋白、尿无机盐以及溶菌酶含量的影响。设计:随机区组分组设计,设立对照实验。单位:新乡医学院药物研究室。材料:实验于2003-09/12在新乡医学院完成。选用健康雌性SD大鼠24只。按区组随机法分组法将大鼠分为3组:正常对照组,糖尿病模型组,糖尿病+鸡冠花黄酮组,每组8只。方法:①糖尿病+鸡冠花黄酮组和糖尿病模型组大鼠腹腔注射链脲佐菌素60mg/kg,48～72h后尾静脉采血,测定全血血糖,血糖≥16.7mmol/L确定为糖尿病大鼠,血糖未达16.7mmol/L者立即腹腔补注链脲佐菌素60mg/kg。造模后糖尿病+鸡冠花黄酮组给予鸡冠花黄酮100mg/(kg·d)灌胃,正常对照组仅注射等量生理盐水。②各组干预10周后,采用原子吸光光度法测定大鼠尿钙含量,火焰分光光度法测尿钠、钾含量。③采用过氧化物酶标记的链霉卵白素法按照说明书进行骨形成蛋白2免疫组织化学测定,用HPIAS-2000图像分析仪对各组骨形成蛋白2免疫组织化学表达进行定量分析。④采用微量溶菌酶比色法测定反映肾小管的重吸收功能的溶菌酶含量。⑤组间差异比较用t检验。主要观察指标:各组干预10周后,大鼠尿钙、钠、钾及溶菌酶含量及骨形成蛋白2免疫组织化学表达比较。结果:SD大鼠24只均进入结果分析。①糖尿病模型组大鼠的尿钙、钠含量明显高于正常对照组,尿钾显著低于正常对照组(P<0.05～0.01)。糖尿病+鸡冠花黄酮组大鼠尿钙含量明显低于糖尿病模型组(P>0.05)。②糖尿病+鸡冠花黄酮组和正常对照组大鼠骨组织骨形成蛋白2表达明显高于糖尿病模型组,尿溶菌酶含量明显低于糖尿病模型组(P<0.05～0.01)。结论:糖尿病大鼠骨组织骨形成蛋白2表达明显下降,补充鸡冠花黄酮类化合物后动物骨骨形成蛋白2表达升高,糖尿病大鼠的尿钙和尿钠的排出显著降低,肾小管的重吸收功能提高。
BACKGROUND: Celosia flavonoids are phytoestrogens with multiple physiological functions and no toxic or side effects. They have good intervention effects on the treatment and prevention of osteoporosis. OBJECTIVE: To investigate the effect of flavonoids from Celosia henryi on bone morphogenetic proteins, urinary inorganic salts and lysozyme contents in diabetic rats. Design: Randomized zone component design, setting up a controlled experiment. Unit: Drug Research Department, Xinxiang Medical College. MATERIALS: The experiment was completed at Xinxiang Medical College from September 09 to December 12. Twenty-four healthy female SD rats were selected. Rats were divided into 3 groups according to the randomized group method: normal control group, diabetic model group, diabetes + Celosia flavonoid group, 8 in each group. METHODS: 1 Diabetic + Celosia flavone group and diabetic model group rats were intraperitoneally injected with streptozotocin 60 mg/kg, and blood was collected from the tail vein 48 to 72 hours. Whole blood glucose was measured and blood glucose ≥ 16.7 mmol/L was determined as diabetic rats. Blood glucose below 16.7 mmol/L was immediate intraperitoneal injection of 60 mg/kg streptozotocin. After modeling, diabetics + Celosia flavonoid group was given intragastric flavonoids at a dose of 100 mg/(kg·d), and only normal saline was injected into the normal control group. 2 After 10 weeks of intervention in each group, the urinary calcium content was measured by atomic absorption spectrophotometry, and the urine sodium and potassium contents were measured by flame spectrophotometry. 3 The expression of bone morphogenetic protein 2 immunohistochemistry was performed according to the instructions using the peroxidase-labeled streptavidin method. The immunohistochemical expression of bone morphogenetic protein 2 in each group was quantitatively analyzed using the HPIAS-2000 image analyzer. 4 The lysozyme content reflecting the reabsorption function of the renal tubules was determined using a micro lysozyme colorimetric method. Differences between the five groups were compared using the t-test. MAIN OUTCOME MEASURES: The levels of urinary calcium, sodium, potassium and lysozyme and the expression of bone morphogenetic protein 2 immunohistochemistry in rats after 10 weeks of intervention were compared. RESULTS: All 24 SD rats were involved in the result analysis. 1 The urinary calcium and sodium levels in the diabetic model group were significantly higher than those in the normal control group, and urinary potassium was significantly lower than that in the normal control group (P<0.05-0.01). The content of urinary calcium in diabetics and celosia flavone group was significantly lower than that in diabetic model group (P>0.05). 2The expression of bone morphogenetic protein 2 in bone tissue of diabetes mellitus + Celosia flavone group and normal control group was significantly higher than that of diabetic model group. The content of urinary lysozyme was significantly lower than that of diabetic model group (P<0.05-0.01). Conclusion: The expression of bone morphogenetic protein 2 in bone tissue of diabetic rats was significantly decreased. The expression of bone morphogenetic protein 2 was increased after flavonoids supplementation. The excretion of urinary calcium and urinary sodium in diabetic rats was significantly reduced, and the weight of renal tubules was decreased. Absorption improves.