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目的 探讨损伤后周围神经产生的活性因子对活体成年青蛙断神经再生轴突的定向趋化诱导作用。方法 通过微型渗透压泵以不同的角度和距离朝被切断的支配左皮胸肌的神经末端提供坐骨神经条件性培养基(conditionedmedium ,CM) 30～ 10 0kD组分 ,形成浓度梯度。 2 8～ 32d后 ,取出胸部组织行免疫组织化学染色示再生的皮胸肌神经轴突 ,并分析再生轴突定向生长的导向值 (directionalvalue)。 结果 再生的皮胸肌神经轴突明显地朝向微型泵管口CM浓度梯度高处生长 ,导向值为 0 84。对照组再生的神经轴突呈无定向生长 ,导向值为 0 0 4。两组间有显著性差异 (P <0 0 0 1)。管口与神经断端间距离和夹角的大小变化对导向值无显著性影响。 结论 损伤后的周围神经可产生分子量介于 30～ 10 0kD可弥散活性因子 ,对活体成年青蛙断神经的再生轴突有定向诱导作用
OBJECTIVE: To investigate the chemotaxis-induced induction of nerve regeneration induced by peripheral nerve in adult adult frog after injury. Methods Microponic osmotic pressure pump was used to provide 30 ~ 100 kD of conditioned medium (CM) to the severed left pectoral muscle at different angles and distances to form a concentration gradient. After 8 ~ 32 days, the thoracic tissues were removed and the regenerated pectoral axons were removed by immunohistochemical staining. The directional value of directional axon regeneration was analyzed. Results The regenerated cords of the pectoral muscle axon apparently grew toward the CM gradient of the micropipe orifice at a height of 0 84. The regenerated axons of the control group showed a non-directional growth with a guide value of 0 0 4. There was a significant difference between the two groups (P <0.01). There was no significant effect of the change of the distance between the orifice and the nerve end and the angle between the orifice and the guide. Conclusion After injury, the peripheral nerve can produce diffusible active factors with molecular weights of 30 ~ 100 kD and induce the axon regeneration of the adult frog