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目的:研究二甲基黄青霉素抑制人肿瘤细胞增殖的作用机制。方法:用MTT法检测细胞增殖的抑制作用,流式细胞仪检测细胞周期分布以及细胞内活性氧自由基的水平,Western blotting检测凋亡相关蛋白的表达。结果:二甲基黄青霉素明显地抑制人肝癌HepG2细胞、人乳腺癌MCF-7细胞和人口腔上皮癌KB细胞的增殖,其IC50值分别为0.18,0.38,0.44μg.mL-1。进一步研究发现:二甲基黄青霉素使细胞阻滞在G2/M期,增加细胞内活性氧自由基水平,激活细胞凋亡通路。结论:二甲基黄青霉素具有明显的抑制肿瘤细胞增殖的作用,其机制与诱导细胞凋亡有关。
Objective: To study the mechanism of dimethylcycline penicillin inhibiting the proliferation of human tumor cells. Methods: The inhibition of cell proliferation was detected by MTT assay. The cell cycle distribution and the level of reactive oxygen species (ROS) in cells were detected by flow cytometry. The expression of apoptosis related protein was detected by Western blotting. Results: Methylparaben significantly inhibited the proliferation of human hepatocellular carcinoma HepG2 cells, human breast cancer MCF-7 cells and human oral epithelial cancer KB cells with IC50 values of 0.18,0.38,0.44μg.mL-1, respectively. Further study found that: dimethyl xanthine blocks cells in the G2 / M phase, increase intracellular reactive oxygen species level, activate the apoptotic pathway. Conclusion: There is obvious effect of xanthine on the proliferation of tumor cells. The mechanism is related to the induction of apoptosis.