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目的:观察钩藤散对阿尔茨海默病(AD)模型大鼠海马CA1区相关指标的影响。方法:用β-淀粉样蛋白(Aβ1-40)海马注射建立AD大鼠模型,然后分成钩藤散高、中、低剂量、模型、正常对照5个组,钩藤散分别用5,2.5,1.25 g.kg-1ig AD大鼠,每天1次,连续给药15 d,14 d时迷宫法测定其行为学指标,未次给药后1 h将动物麻醉、心脏灌注、取脑、制作海马CA1区病理切片,用免疫组化法测定神经细胞数,Aβ,突触后密度蛋白-95(PSD-95)阳性细胞数和平均吸光度(A)。结果:行为学测试成绩:对照组、各给药组均好于模型组(P<0.01),说明模型制作成功并提示钩藤散有益智作用;CA1区的神经细胞数、PSD-95阳性细胞数和平均A:各给药组均高于模型组(P<0.001);Aβ阳性细胞数和平均A:各给药组均低于模型组(P<0.01),说明钩藤散能有效降低脑组织Aβ含量、提高PSD-95水平,保护神经细胞。结论:钩藤散对AD模型大鼠海马组织有一定保护作用。
Objective: To observe the effects of Gouteng San on the related indexes of hippocampus CA1 area in Alzheimer’s disease (AD) model rats. Methods: The rat model of AD was established by injecting β-amyloid protein (Aβ1-40) into hippocampus and then divided into 5 groups: Uncaria, medium and low dose, model and normal control. 1.25 g.kg-1ig AD rats were given once daily for 15 days. The behavioral indexes were determined by maze method on the 14th day. Animals were anesthetized and perfused 1 hour after the second administration, and the hippocampus The number of neurons, Aβ, PSD-95 positive cells and average absorbance (A) were measured by immunohistochemistry. Results: The results of behavioral test: the control group and each administration group were better than the model group (P <0.01), indicating that the model was made successfully and prompted the Uncaria random puzzle; CA1 area of nerve cells, PSD-95 positive (P <0.001). The number of Aβ positive cells and the average A were lower in each administration group than in model group (P <0.01), indicating that Uncaria was effective Reduce Aβ content in brain tissue, increase PSD-95 level and protect nerve cells. Conclusion: Gouteng San has certain protective effect on hippocampus of AD model rats.