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目的 了解肝癌的基因表达概况 ,寻找肝癌及癌旁肝组织中差异表达基因。方法以 2 4例肝癌及癌旁肝组织的总RNA反转录合成含有α 32 PdATP的cDNA为探针 ,与Atlas微阵列表达分析膜进行差异杂交。结果 放射自显影结果经AtlasImageTM软件分析显示 :在所分析的 5 88种已知基因中 ,TFDP2、Akt1、E2F 3等 18个在肝癌组织中表达上调 ,TDGF1、BAK、LAR等 2 5个表达下调 ,参与细胞增殖、凋亡、分化、细胞间相互作用、与细胞侵袭相关的基因表达水平发生了明显改变。结论 通过Atlas微阵列系统分析发现的这些基因的表达改变组成了一个肝癌特异的基因表达谱 ,系统地研究肝癌的基因表达改变 ,与肝癌发生相关的差异变化基因可为肝癌早期诊断和治疗提供线索。
Objective To understand the gene expression profile of liver cancer and to find differentially expressed genes in liver cancer and adjacent liver tissues. Methods The cDNA containing α 32 PdATP was synthesized by reverse transcription of total RNA from 24 hepatocellular carcinomas and adjacent liver tissue. The cDNA was hybridized with Atlas microarray expression analysis. Results The results of autoradiography were analyzed by AtlasImageTM software. Among the 5 88 known genes analyzed, 18 were up-regulated in hepatoma tissues such as TFDP2, Akt1, and E2F3, and 25 expressions of TDGF1, BAK, and LAR were down-regulated. Involved in cell proliferation, apoptosis, differentiation, cell-cell interactions, and gene expression levels associated with cell invasion have changed significantly. Conclusion The expression changes of these genes identified by Atlas microarray analysis constitute a hepatocellular carcinoma-specific gene expression profile, systematically studying the gene expression changes of liver cancer, and the differentially altered genes associated with the occurrence of liver cancer can provide clues for the early diagnosis and treatment of liver cancer. .