论文部分内容阅读
目的:在体外环境下观察基因重组人生长激素(rhGH)及联合氟尿嘧啶(5-FU)干预不同细胞膜生长激素受体(GHR)表达水平对人结肠癌细胞的增殖情况。方法:采用免疫细胞化学法检测人结肠癌细胞株HCT-8和LOVO膜表面的GHR表达水平。每株细胞分为对照组、rhGH组、5-FU组和rhGH+5-FU组,用四甲基偶氮唑蓝(MTT)、克隆形成实验和免疫细胞化学等方法,测定各组各株肿瘤细胞的抑制率、增殖细胞核抗原(PCNA)表达变化。结果:HCT-8高表达GHR(阳性表达细胞数≥50%),LOVO未表达GHR。rhGH显著提高HCT-8细胞增殖和克隆形成率,PCNA表达增加,且联合化疗后,较LOVO组肿瘤抑制率减少(P<0.05)。结论:在体外环境下,rhGH促GHR阳性表达的结肠癌细胞增殖,且对5-FU化疗有抵抗,对于不表达GHR的结肠癌细胞无上述作用。
OBJECTIVE: To observe the effects of rhGH and 5-FU on the proliferation of human colon cancer cells in vitro under different levels of GHR expression. Methods: Immunocytochemistry was used to detect the expression of GHR on human colon cancer cell lines HCT-8 and LOVO. Each group of cells was divided into control group, rhGH group, 5-FU group and rhGH + 5-FU group. MTT assay, colony formation assay and immunocytochemistry were used to determine the effect of each strain Tumor cell inhibition rate, proliferating cell nuclear antigen (PCNA) expression change. Results: HCT-8 overexpression GHR (positive cells ≥ 50%), LOVO did not express GHR. rhGH significantly increased the proliferation and colony formation of HCT-8 cells, and increased the expression of PCNA. Compared with LOVO group, the tumor inhibition rate decreased (P <0.05). CONCLUSION: In vitro, rhGH promotes the proliferation of colon cancer cells with positive GHR expression, and is resistant to 5-FU chemotherapy. It does not have the above-mentioned effects on colon cancer cells that do not express GHR.