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定量确定基因表达水平的方法主要有实时定量PCR和相对定量检测。一般只有在需要确定基因的拷贝数时,才利用实时定量PCR检测。目前大部分的研究中还是选择相对定量的检测。相对定量检测有Northernblot和相对定量PCR两种检测方法,均需要采用内标来确定目标基因的相对表达量。
Quantitative methods to determine the level of gene expression are real-time quantitative PCR and relative quantitative detection. Generally only when it is necessary to determine the copy number of a gene, real-time quantitative PCR is used. Most of the current study or choose a relatively quantitative test. Relative quantitative detection of Northernblot and relative quantitative PCR two detection methods, need to use the internal standard to determine the relative expression of the target gene.