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目的 观察中药复方柴胡疏肝散、四君子汤对CD4+ T细胞活化信号蛋白CaN转录表达变化 ,探讨健脾益气、疏肝理气方药免疫调节机制。方法 采用Ova作为抗原诱导 ,制备中药含药血清 ,干预体外培养的Th细胞 ,RT -PCR、Westernblotting检测CaN的转录和表达。结果 发现CaN转录表达在CD4+ T细胞活化后明显增强 ,柴胡疏肝散、四君子汤血清均可以使其有意义的降低 ,而柴胡疏肝散尚可使表达不足的CaN增加。结论 益气、理气中药复方通过影响CD4+ T细胞活化分化过程中不同的信号蛋白转录表达调节Th1/Th2平衡 ,对Th2介导的免疫性疾病 ,理气方药优于益气方药。
Objective To observe the changes of transcriptional expression of CaN of CD4+ T cell activation signal by traditional Chinese medicine compound Chaihu Shugan San and Sijunzi Decoction, and to explore the mechanism of immune regulation of Jianpi Qiqi and Shuganliqi prescription. Methods Ova was used as antigen-inducing agent to prepare serum containing traditional Chinese medicine and interfere Th cells in vitro. The transcription and expression of CaN were detected by RT-PCR and Western blotting. Results It was found that the transcription of CaN was significantly increased after activation of CD4+ T cells. The serum of Chaihu Shugansan and Sijunzi decoction can significantly reduce it, but Chaihu Shugan San can also increase the expression of insufficient CaN. Conclusion Traditional Chinese herbal formulas for replenishing qi and Qi regulate the balance of Th1/Th2 by affecting the transcriptional expression of different signaling proteins during activation and differentiation of CD4+ T cells. For Th2-mediated immune diseases, qi prescription is better than Yiqi prescription.