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[目的]建立谷子突变体库,以便在谷子分子生物学研究中应用。[方法]以生产上主要推广种植的谷子品种‘长农35号’干种子为实验材料,采用0.8%和1.0%甲磺酸乙酯(EMS)进行了诱变处理,并对所得突变体成熟期株型的7个表型形状进行考察,并据此对突变体进行分类。[结果]试验共收获株型相关的M1代突变体材料共282份,其中,0.8%EMS诱变‘长农35号’获得株型突变体材料100个单株,可分为10个组;1.0%EMS诱变‘长农35号’获得株型突变体材料182个单株,可分为17个组。M1代成熟期株型相关性状突变体分析结果表明:1.0%EMS处理‘长农35号’所获突变体,在株高、穗下节粗、穗下第一节间粗和茎节数4个性状与对照有显著差异,而0.8%处理与对照差异不显著。[结论]对于‘长农35号’来讲,采用1.0%EMS进行诱变处理更有利于多类型大量突变单株的获得。
[Objective] The research aimed to establish a library of millet mutants for application in the study of molecular biology of millet. [Method] With dry seeds of ’Changnong 35’, a major popularized millet variety in production, as experimental materials, the mutants were treated with 0.8% and 1.0% ethyl methanesulfonate (EMS), and the resulting mutants were matured Seven plant phenotypes were investigated, and mutants were classified accordingly. [Result] A total of 282 M1 mutant plants were obtained. Among them, 100 mutant plants were obtained from 0.8% EMS mutant ’Changnong 35’ and could be divided into 10 groups. 1.0% EMS mutagenesis ’Changnong 35’ obtained 182 plant mutant strains, which can be divided into 17 groups. The results showed that the mutants of ’Miannong 35’ with 1.0% EMS treatment had higher plant height, lower panicle diameter and panicles, There was a significant difference between the traits and the control, while the difference between the 0.8% treatment and the control was not significant. [Conclusion] For ’Changnong 35’, the mutagenesis treatment with 1.0% EMS was more beneficial for obtaining a large number of mutant plants with many types.