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采用CTAB改良法提取葡萄属 7种植物的基因组DNA ,对其完整性进行了测定 ,并以此为模板进行RAPD反应 .对RAPD反应体系的 5个主要影响因子进行系统的初选与复选 ,获得了葡萄属植物的RAPD最优反应体系及扩增程序 .
The genomic DNA of seven species of Vitis vinifera L. was extracted by CTAB modified method, and its integrity was determined and used as a template for RAPD reaction.Five main influencing factors of RAPD reaction system were systematically selected and checked, The optimal RAPD reaction system and amplification program of the genus Vitis were obtained.