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目的比较三级别宫颈上皮内瘤变(CIN)组织标本中人乳头瘤病毒(HPV)感染的基因型分布情况及其临床意义。方法采用聚合酶链式反应和基因芯片检测技术对20例宫颈正常组织、185例宫颈CIN1级、98例宫颈CIN2级和118例宫颈CIN3级患者宫颈组织标本进行23种HPV基因分型检测,并对受检者进行相关资料分析。结果 20例宫颈正常组织检出HPV感染者2例,总的HPV感染率为10.00%(2/20);185例宫颈CINⅠ组织检出HPV感染者83例,总的HPV感染率为44.87%(83/185);98例宫颈CINⅡ组织检出HPV感染者83例,总的HPV感染率为84.69%(83/98);118例宫颈CIN Ⅲ组织检出HPV感染者108例,总的HPV感染率为91.53%(108/118)。结论聚合酶链式反应结合基因芯片技术可应用于宫颈上皮内瘤变组织标本的HPV基因分型检测,对我国女性宫颈病变HPV感染基因型分布的研究及宫颈癌瘤的防治及其疫苗的研发具有十分重要的意义。
Objective To compare the genotypes of human papillomavirus (HPV) infection in three levels of cervical intraepithelial neoplasia (CIN) and its clinical significance. Methods Twenty-three HPV genotypes were detected by polymerase chain reaction and microarray in 20 cases of cervical normal tissue, 185 cases of cervical CIN1 grade, 98 cases of cervical CIN2 grade and 118 cases of cervical CIN3 grade. The subjects were related data analysis. Results Two cases of HPV infection were detected in 20 cases of normal cervical tissue, the total HPV infection rate was 10.00% (2/20). In 185 cases of cervical CIN Ⅰ tissue, 83 cases were detected, the total HPV infection rate was 44.87% 83 cases of HPV infection were detected in 98 cases of cervical CINⅡ tissue, the total HPV infection rate was 84.69% (83/98); 118 cases of cervical CIN Ⅲ tissues were detected 108 cases of HPV infection, the total HPV infection The rate was 91.53% (108/118). Conclusion Polymerase chain reaction combined with gene chip technology can be applied to HPV genotyping of cervical intraepithelial neoplasia samples. The genotypic distribution of HPV infection in Chinese women with cervical lesions and the prevention and treatment of cervical cancer and its vaccine development It is of great significance.