为使核磁共振谱技术应用于药物代谢产物的研究，用固相萃取核磁共振谱法对药物溴莫普林体内代谢产物进行了解析。用固相萃取法将大鼠尿液中的内源性物质与药物代谢产物分离后，经梯度洗脱和１Ｈ核磁共振谱测定，建立了对药物代谢产物混合物图谱的解析方法。结果成功地鉴定出５种主要的代谢产物，代谢产物的类型与文献报道相同。提示本测定方法有省时、省力的优点，并避免分离、提纯代谢产物时引起结构改变 In order to make use of NMR spectroscopy in the study of drug metabolites, the metabolites of brimolam in the body were analyzed by solid-phase extraction-NMR spectroscopy. After the separation of endogenous substances and drug metabolites in rat urine by solid-phase extraction, the analytical method of the mixture of drug metabolites was established by gradient elution and 1H nuclear magnetic resonance spectroscopy. Results Five major metabolites were successfully identified. The types of metabolites were the same as reported in the literature. Tip This method has the advantages of time-saving, labor-saving, and to avoid separation, purification of metabolites caused by structural changes