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本研究以珊瑚石为培养载体,分析海水中无机氮与活性磷酸盐的变化、微生物膜的形态和底栖硅藻的种类,并比较附苗器上微生物膜培养时间对大珠母贝幼虫附着率的影响。结果显示:添加营养盐时可溶性无机氮和活性磷酸盐质量浓度均随时间逐渐下降,至16 d基本稳定,分别较初始值减少了99.11%和84.78%,而未添加营养盐时可溶性无机氮和活性磷酸盐质量浓度均无明显变化;添加营养盐的海水中培养了17 d的珊瑚石表面附着的硅藻包括菱形藻属、圆筛藻属、双眉藻属等,与球状细菌、胞外分泌物等共同形成微生物膜;未添加营养盐的海水中培养了17 d的珊瑚石表面附着的硅藻主要有异极藻属、胸隔藻属和曲壳藻属等,微生物膜结构相对疏松,珊瑚石孔洞间几乎没有微生物膜;在添加营养盐的微生物膜培养水体中处理4 d的附苗器48 h大珠母贝附苗率为25.78%,较对照组提升了9.87%,且显著高于处理2 d和3 d的附苗器附苗率。因此,自然海水中可以培育形成底栖硅藻和细菌微生物膜,促进大珠母贝幼虫附着。
In this study, the coral stone culture carrier, analysis of seawater inorganic nitrogen and active phosphate changes in microbial membrane morphology and species of benthic diatoms, and compared with the attached device on the microbial culture membrane larvae attached to large pearl Rate of impact. The results showed that the contents of soluble inorganic nitrogen and active phosphate decreased gradually with the increase of nutrient salts, and remained stable at 16 days, decreasing by 99.11% and 84.78% respectively compared with the initial values. However, the contents of soluble inorganic nitrogen and soluble inorganic nitrogen There was no significant change in the mass concentration of active phosphate. The diatoms attached to the surface of coral in the seawater supplemented with nutrient salt for 17 days included Rhodophycea, Cnidium, Abrus, etc., and globular bacteria, extracellular secretion Etc. formed the microbial membrane together. The diatoms attached to the surface of the coral on the 17th day after cultured in seawater without nutrients were mainly Algae, Pleuropneumonia, and Tragacanth. Microbial membrane structure was relatively loose, There was almost no microbial membrane between coralline holes. The rate of the attached seedlings was 25.78% in 48 h after the 4-day incubation with the addition of nutrient salt, which was 9.87% higher than that of the control group In the treatment of 2 d and 3 d attached vaccine attached rate. As a result, benthic diatoms and bacterial microbial membranes can be bred in natural seawater to promote larvae adhering to Larvae.